| 稀碱法提取酵母RNA的正交试验研究 |
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| 引用本文： | 张淑桂,钱敏.稀碱法提取酵母RNA的正交试验研究[J].云南化工,1994(2):10-12. |
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| 作者姓名： | 张淑桂 钱敏 |
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| 作者单位： | 云南大学实验中心，云南民族学院化学系，云南大学图书馆 |
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| 摘 要： | 采用正交设计试验法对稀碱法提取酵母ＲＮＡ工艺过程中的菌悬液浓度、稀碱浓度、反应时间和沉淀剂用量等因素进行了研究。用方差分析处理实验结果表明，１０％酵母浓度、１．０％碱、２．５％氯化钙和６０分钟反应时间是提取酵毒ＲＮＡ的适宜条件，并将其用于啤酒厂废酵母泥的ＲＮＡ提取试验。
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| 关 键 词： | 稀碱法，提取，酵母RNA，正交试验 |
Study on Optimization Test or Isolation of Yeast RNA by Dilute Alkali Method |
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| Zhang Shugui, Qian min,Wang Guangcan,Dong Wen-ying,Zheng Zhiying.Study on Optimization Test or Isolation of Yeast RNA by Dilute Alkali Method[J].Yunnan Chemical Technology,1994(2):10-12. |
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| Authors: | Zhang Shugui Qian min Wang Guangcan Dong Wen-ying Zheng Zhiying |
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| Abstract: | The process of isolation yeast RNA by the dilute alkali method The influence factors Which are the concentration of yeast and alkali as well as reaction time etc.were investigated by means of the optimization test. The experiment results analyzed by the analysis of variance indicate that the yeast of 10%. the alikali of 1.0%, the calcium chlorid of 2.5% and the reaction time of 60 minutes were the suitable conditions for the isolation of yeast RNA. These were applied in the isolation RNA from waste yeast in beer factory. |
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| Keywords: | Dilute alkali method Isolation Yeast RNA Optimiazation test |
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