Distribution of deuterium-labeledcis-andtrans-12-octadecenoic acids in human plasma and lipoprotein lipids |
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| Authors: | E A Emken H J Dutton W K Rohwedder Henry Rakoff R O Adlof R M Gulley J J Canary |
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| Affiliation: | (1) Northern Regional Research Center, Agricultural Research Science and Education Administration, U.S. Department of Agriculture, 61604 Peoria, IL;(2) St. Francis Hospital-Medical Center, 61637 Peoria, IL;(3) School of Medicine, Georgetown University, 20007 Washington, DC |
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| Abstract: | Triglycerides containingcis- andtrans-12-octadecenoic acid (12c-18∶1 and 12t-18∶1) andcis-9-octadecenoic acid (9c-18∶1) labeled with deuterium were fed to 2 young adult male subjects. These fatty isomers each contained a different number
of deuterium labels, which allowed mass spectrometric analysis to distinguish among them after they were fed as a mixture.
This approach results in a direct comparison of the absorption and distribution of these 3 monoenoic acids into blood plasma
and lipoprotein lipids. Plasma lipid data indicated that all phospholipid fractions selectively incorporate 12c-18∶1 and 12t-18∶1 in preference to 9c-18∶1. Discrimination against 12c-18∶1 and 12t-18∶1 compared to 9c-18∶1 was found in the plasma neutral lipids, with a strong discrimination against 12t-18∶1 incorporation into the cholesteryl ester fraction. Considerable reduction in the percentage of linoleic and arachidonic
acid was observed when 12–18∶1 isomers were incorporated in plasma triglyceride, phosphatidylcholine and sphingomyelin samples.
Chylomicron lipid analyses indicated that all isomers were well absorbed. Variation was observed in the relative distribution
of 12c-18∶1, 12t-18∶1 and 9c-18∶1 between the very low density, low density and high density lipoprotein lipid classes. No desaturation of 12c-18∶1 to linoleic acid was detected. |
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