|
|||||
|
|
DNA ligation using a disposable microfluidic device combined with a micromixer and microchannel reactor |
|
| Authors: | Yong-Jun KoAuthor Vitae Joon-Ho MaengAuthor Vitae Yoomin AhnAuthor Vitae Seung Yong HwangAuthor Vitae |
| Affiliation: | a Department of Mechanical Engineering, Graduate School, Hanyang University, Seoul 133-791, Republic of Korea b Department of Biochemistry, Graduate School, Hanyang University, Ansan, Gyeonggi 426-791, Republic of Korea c Department of Mechanical Engineering, Hanyang University, 1271 Sa-3-dong, Sangnok-gu, Ansan, Gyeonggi 426-791, Republic of Korea d Division of Molecular and Life Science, Hanyang University, and GenoCheck Co. Ltd., Ansan, Gyeonggi 426-791, Republic of Korea |
| Abstract: | A novel PDMS and glass-based microfluidic device consisting of a micromixer and microreactor for DNA ligation is described in this article. The new passive type planar micromixer is 10.33 mm long and composed of a straight channel integrated with nozzles and pillars, and the microreactor is composed of a serpentine channel. Mixing was enhanced by convective diffusion facilitated by the nozzles and pillars. The performance of the micromixer was analytically simulated and experimentally evaluated. The micromixer showed a good mixing efficiency of 87.7% at a 500 μL/min flow rate (Re = 66.5). DNA ligation was successfully performed using the new microfluidic device, and ligation time was shortened from 4 h to 5 min. When used for on-chip ligation, this new micromixer offers advantages of disposability and portability. |
| Keywords: | DNA ligation Micromixer Micropillar Micronozzle Serpentine microreactor PDMS-glass microbiochip |
| 本文献已被 ScienceDirect 等数据库收录！ | |