Antioxidant Activity of Tannin Fractions Isolated from Buckwheat Seeds and Groats |
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Authors: | Magdalena Karama? |
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Affiliation: | (1) Division of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland |
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Abstract: | Phenolic compounds were extracted with 80% (v/v) aqueous acetone from buckwheat seeds and groats. Tannin fractions were obtained
from the crude extracts by Sephadex LH-20 column chromatography. Total phenolic contents of isolated fractions from buckwheat
seeds and groats were 477 and 371 mg catechin equiv/g, respectively. The analyzed samples were characterized by electrophoretic
separations using capillary zone electrophoresis. Both fractions exhibited strong antioxidant activity. The tannin fraction
of buckwheat seeds reduced 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH·) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] diammonium salt radical cations (ABTS·+) stronger than the fraction of buckwheat groats. The EC50 values amounted to 0.019 and 0.020 mg while Trolox equivalent antioxidant capacity values were 4.06 and 3.55 mmol Trolox
equiv/g for tannin fractions of buckwheat seeds and groats, respectively. Similarly, antioxidant activity of the tannin fraction
of buckwheat seeds measured by photochemiluminescence assay, was higher than antioxidant activity noted for the fraction obtained
from groats. However, both fractions inhibited oxidation to the same extent in applied lipid models: β-carotene-linoleic acid
emulsion and l-α-lecithin liposomes. In β-carotene-linoleic acid emulsion system, 1 mg of tannin fractions exhibited similar antioxidant
activity to 0.5 mg butylated hydroxyanisole (BHA). |
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