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Real-time two-photon confocal microscopy using a femtosecond, amplified Ti:sapphire system |
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| Authors: | G J BRAKENHOFF J SQUIER T NORRIS A C BLITON M H WADE & B ATHEY |
| Affiliation: | Confocal Microscopy Group, Department of Molecular Cell Biology, University of Amsterdam, Pl. Muidergr. 14, 1018 TV, Amsterdam, The Netherlands;, Centre for Ultrafast Optical Science, University of Michigan, 2200 Bonisteel, IST Bldg RM 1006, Ann Arbor, MI 48109-2099, U.S.A.;, Meridian Instruments, Inc., 2310 Science Parkway, Okemos, MI 48864, U.S.A.;, Department of Anatomy and Cell Biology, University of Michigan, Medical School, Ann Arbor, MI 48109-0616, U.S.A. |
| Abstract: | The bilateral imaging approach known from confocal applications operating in the line mode was used to realize real-time two-photon imaging. It is shown that the sectioning inherent to two-photon imaging could be improved by the introduction of a confocal line aperture in the imaging path. Using a high-power, low-repetition-rate amplified Ti:sapphire system, various biological objects were visualized including live boar sperm. |
| Keywords: | Bilateral confocal real-time imaging sectioning three-dimensional imaging TPA two-photon fluorescence |