放线菌AF1发酵液提取物对病原微生物的抑菌作用和稳定性分析

对放线菌菌株AFI乙酸乙酯提取物的抑菌谱做了研究。抑菌活性试验表明:AF1发酵液提取物对8种植物病原真菌和10种革兰氏阳性病原菌都具有抑制活性,对4种革兰氏阴性致病菌无抑制作用。在供试的8种植物病原菌中,放线菌AF1发酵液提取物对香蕉枯萎病菌(fusarium oxysporum f.sp.cubense)和苦瓜枯萎病菌(fusarium oxysporum f.sp.momodicae)的抑菌率达到85%以上。稳定性试验结果表明:菌种AF1发酵液对紫外线、热和酸碱较稳定,室温至80℃时稳定,90℃时活性丧失,在pH 2~10范围内稳定,耐受紫外线照射。试验表明该菌株发酵液提取物对革兰氏阳性致病菌的抑制作用较好,有较强的稳定性,具有一定的开发价值。

Analysis of the Inhibitory Spectrum and Stability of Fermentation Broths of Actinomycete Strain AF1

The inhibitory spectrum and stability of fermentation broths of actinomycete strain AF1 were studied.The result of antimicrobial activity experiment showed that the fermentation broths had significant inhibitory effect on 8 tested species of plant pathogen and 10 tested G＋pathogen, while no effect to G-pathogenic.The fermentation broths shows the strongest antimicrobial effect against Fusarium oxysporum f.sp.cubense and Fusarium oxysporum f.sp.momodicae among 8 fungi species, the inhibitory rate reached up to 85%. The antimicrobial substance was rather stable under the pH 2~10 and ultraviolet radiation, while 90 ℃ for 30 min treatment deprives its antimicrobial activity thoroughly.