牛肝菌生长与发酵主要影响因子的鉴别

采用Plackett-Burman设计法,分别研究了影响牛肝菌(Boletussp.)ACCC50328菌丝生长和发酵产糖的21个相关因素.结果表明,影响牛肝菌ACCC50328菌株菌丝生长速度的主要因子为葡萄糖、麦芽糖、酵母膏、KH2PO4、(NH4)2SO4、FeSO4、发酵温度、发酵时间及装液量;影响该菌株胞外多糖含量的主要因子为麦芽糖、酵母膏、(NH4)2SO4、CuSO4·5H2O、FeSO4和发酵时间.

Determination of the Most Important Factors Affecting the Mycelium Biomass and Exopolysaccharide Yield in Submerged Fermentation

Mycelium biomass and polysaccharide accumulation in submerged fermentation by Boletus sp. ACCC 50328 are strongly influenced by many internal and external factors, namely medium constituents and fermentation conditions. the Plackett-Burman technique was applied in the present investigation to design experiments, build models, and evaluate importance of the twenty-one selected factors. By the statistical regression analysis, the significant factors affecting the mycelium biomass and exopolysaccharide yield in submerged fermentation of Boletus sp. ACCC 50328 were: glucose, maltose, yeast extract, potassium dihydrogen phosphate, ammonium sulfate, ferrous sulfate, temperature, fermentation time and broth content; and maltose, yeast extract, ammonium sulfate, copper sulfate pentahydrate, ferrous sulfate and fermentation time, respectively.