| EGF-PE35KDEL重组毒素的基因构建及表达 |
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| 引用本文： | 李树民,李丽萍,岳玉环,李铁征,邱业峰,朱平. EGF-PE35KDEL重组毒素的基因构建及表达[J]. 中国生物制品学杂志, 2003, 16(2): 68-70 |
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| 作者姓名： | 李树民 李丽萍 岳玉环 李铁征 邱业峰 朱平 |
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| 作者单位： | 中国人民解放军军需大学军事兽医研究所 长春130062(李树民，李丽萍，岳玉环，李铁征，邱业峰)，中国人民解放军军需大学军事兽医研究所 长春130062(朱平) |
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| 摘 要： | 目的 构建表达有特异细胞毒性融合蛋白的工程质粒。方法 利用PCR扩增出PE3 5KDEL基因片段 ,经酶切后插入EGF PET2 8A载体 ,并转化至BL2 1(DE3 )中。采用Westernblot对重组毒素进行鉴定。结果EGF PE3 5KDEL重组毒素占菌体总蛋白的 2 0 % ,分子量约为 410 0 0。结论 EGF PE3 5KDEL的成功构建和表达为开发靶向抗癌药物奠定了基础
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| 关 键 词： | 表皮生长因子 绿脓杆菌外毒素 重组毒素 |
| 修稿时间： | 2002-11-28 |
Construction and Expression of Recombinant EGF-PE35KDEL Toxin Gene |
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| LI Shumin,LI Liping,YUE Yuhuan et al. Construction and Expression of Recombinant EGF-PE35KDEL Toxin Gene[J]. Chinese Journal of Bilogicals, 2003, 16(2): 68-70 |
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| Authors: | LI Shumin LI Liping YUE Yuhuan et al |
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| Abstract: | Objective To construct a engineering plasmid which can express special cytotoxic fusion protein.Methods Amplify PE35KDEL DNA fragment by PCR,digest with restriction endonuclease,insert into EGF PET28a vector and transform to BL21(DE3) for expression under induction of IPTG.The expressed product was identified by Western blot.Results The expressed EGF PE35KDEL toxin contained 20% of total somatic protein,and the relative molecular weight of it was about 41 000.Conclusion The successful construction and expression of EGF PE35KDEL laid a foundation for developing potential targeting antitumor medicine. |
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| Keywords: | Epidermal growth factor Pseudomonas exotoxin Recombinant toxin |
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