Stoichiometric formation of poly-ion complexes between human carboxyhemoglobin and potassium poly(vinyl alcohol) sulfate |
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| Authors: | Etsuo Kokufuta Hidetoshi Shimizu Isei Nakamura |
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| Affiliation: | (1) Institute of Applied Biochemistry, University of Tsukuba, Sakura-mura, Niihari-gun, 300-31 Ibaraki, Japan |
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| Abstract: | Summary Colloid titrations of human carboxyhemoglobin were carried out at different pH. The total number of basic groups in the hemoglobin was 95, as evaluated by the titration with potassium poly (vinyl alcohol) sulfate (KPVS). The value estimated was comparable with that obtained from the amino acid sequence of human hemoglobin. This finding indicates the stoichiometric salt-linkage formation between the basic groups in the hemoglobin and the KOSO3-groups in KPVS. |
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