|
|||||
|
|
| 毛细管电泳测定乳酸发酵液中有机酸 | |
| 引用本文： | 唐萍,田晶,苑广志,高鹏,许国旺.毛细管电泳测定乳酸发酵液中有机酸[J].食品与发酵工业,2006,32(3):76-78. |
| 作者姓名： | 唐萍 田晶 苑广志 高鹏 许国旺 |
| 作者单位： | 1. 中国科学院大连化学物理研究所国家色谱研究分析中心,大连,116011;大连理工大学化工学院,大连,116021 2. 中国科学院大连化学物理研究所国家色谱研究分析中心,大连,116011;大连轻工业学院,大连,116034 3. 中国科学院大连化学物理研究所国家色谱研究分析中心,大连,116011 |
| 基金项目： | 科技部科研项目;中国科学院知识创新工程项目 |
| 摘 要： | 运用毛细管区带电泳模式,在磷酸缓冲液中成功地分离了苹果酸、甲酸、乙酸、丙酸、琥珀酸、酒石酸、柠檬酸、乳酸、草酸9种有机酸。研究了缓冲液浓度、pH值、电泳电压、检测波长等因素对分离的影响,并对实际发酵液进行分析测定,加标回收率为97.5%～107.1%。该方法简便、快速、准确,适于整个发酵过程条件的优化及发酵产物的监控工作。 |
| 关 键 词： | 毛细管电泳 发酵液 有机酸 乳酸 |
| 收稿时间： | 09 26 2005 12:00AM |
| 修稿时间： | 12 12 2005 12:00AM |
Determination of Organic Acid in Fermentation Broth by Capillary Electrophoresis |
|
| Tang Ping,Tian Jing,Yuang Guangzhi,Gao Peng,Xu Guowang.Determination of Organic Acid in Fermentation Broth by Capillary Electrophoresis[J].Food and Fermentation Industries,2006,32(3):76-78. | |
| Authors: | Tang Ping Tian Jing Yuang Guangzhi Gao Peng Xu Guowang |
| Abstract: | The profile of organic acid is investigated by capillary electrophoresis(CE).The nime organic acids (malic acid, formic acid, succinin acid, oxalic acid, tartaric acid, citric acid, acetic acid, propionic acid, lactic acid) of fermentation liquid were well separated by using 500 mmol/L phosphoric acid running buffer. The concentration and pH of the buffer solution,voltage and wave for analytical were investigated in details.The proposed method was applied to the determination of organic acid in fermentation liquid products and the recoveries were in the range of 97.5%～107.1%.This method is simple,rapid,accurate and is suitable for optimizing condition of experiment in the course of ferment and monitoring products of fermentation. |
| Keywords: | capillary electrophoresis fermentation liquid organic acid Lactic acid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |