| 胶体金免疫层析法快速检测猪肉及牛奶中磺胺二甲嘧啶残留 |
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| 引用本文： | 张佳宜,张燕,王硕,.胶体金免疫层析法快速检测猪肉及牛奶中磺胺二甲嘧啶残留[J].中国食品学报,2010,10(1):181-185. |
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| 作者姓名： | 张佳宜 张燕 王硕 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院,教育部食品营养与安全重点实验室,天津,300457 |
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| 基金项目： | "十一五"国家科技支撑计划资助项目 |
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| 摘 要： | 目的:以胶体金为标记物,根据竞争抑制免疫层析原理,建立一种快速检测猪肉及牛奶中磺胺二甲嘧啶残留的方法.方法:采用柠檬酸三钠法制备40nm胶体金.电镜鉴定后标记磺胺二甲嘧啶多克隆抗体:磺胺二甲嘧啶半抗原-OVA和羊抗兔二抗分别包被在硝酸纤维素膜上,金标抗体喷涂在玻璃纤维垫上,制成免疫层析快速检测试纸条.结果:所建立的检测体系方法检出限为1 μg/L,将该法与高效液相色谱法(HPLC)对比检测猪肉和牛奶样品,在试纸务的检测范围内,与HPLC法检测结果一致性好,从而验证了该方法的有效性.结论:通过消除基质影响,成功检测到猪肉及牛奶中的磺胺二甲嘧啶残留.该方法灵敏度高,样品前处理简便、快速,整个检测过程可在10min内完成.该方法适用于动物源性食品中磺胺二甲嘧啶残留的大批量现场检测.
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| 关 键 词： | 磺胺二甲嘧啶 胶体金 免疫层析试纸条 猪肉 牛奶 |
Rapid Detection of Sulphamethazine Residues in Pig Muscle and Milk by Colloidal Gold-based Immumochromatographic Assay |
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| Zhang Jiayi,Zhang Yan,Wang Shuo.Rapid Detection of Sulphamethazine Residues in Pig Muscle and Milk by Colloidal Gold-based Immumochromatographic Assay[J].Journal of Chinese Institute of Food Science and Technology,2010,10(1):181-185. |
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| Authors: | Zhang Jiayi Zhang Yan Wang Shuo |
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| Affiliation: | Zhang Jiayi Zhang Yan Wang Shuo(Key Laboratory of Food Nutrition , Safety,Ministry of Education,Faculty of FoodEngineering , Biotechnology,Tianjin University of Science , Technology,Tianjin 300457) |
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| Abstract: | Objective: The objective of the study is to establish a competitive assay based on the principle of immunoassay using colloidal gold as tracer for the rapid on-site detection of Sulphamethazine(SMZ) residues in pig muscle and milk.Methods: Tri-sodium citrate with aqueous gold chloride were mixed to make 40 nm colloidal gold which werelabeled to the anti-SMZ polyclonal antibody after identification by electron micrograph.The SMZ-OVA and goat anti-rab-bit IgG were dispense on test line and control line respec... |
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| Keywords: | SMZ colloidal gold immunochromatographic test strip pig muscle milk |
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