| 氨氯地平对人乳腺癌细胞MCF-7细胞周期和周期蛋白表达的影响及其调控机制 |
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| 引用本文： | 黄文静,李卫平,孙文娟. 氨氯地平对人乳腺癌细胞MCF-7细胞周期和周期蛋白表达的影响及其调控机制[J]. 中国生物制品学杂志, 2010, 23(3) |
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| 作者姓名： | 黄文静 李卫平 孙文娟 |
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| 作者单位： | 重庆市生物化学与分子药理学重点实验室,重庆医科大学药学院药理教研室,重庆,400016 |
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| 基金项目： | 重庆医科大学自然科学基金 |
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| 摘 要： | 目的探讨氨氯地平对人乳腺癌细胞MCF-7细胞周期及周期蛋白表达的影响及其调控机制。方法以不同浓度的氨氯地平处理对数生长期的MCF-7细胞,MTT法检测细胞增殖水平;流式细胞仪分析细胞周期;免疫细胞化学法检测细胞周期蛋白cyclinD1、p21和p53的表达。结果氨氯地平呈剂量和时间依赖性抑制MCF-7细胞增殖,IC50为14.439μmol/L;经7.220μmol/L(0.5IC5)0、14.439μmol/L(1IC5)0和28.880μmol/L(2IC50)氨氯地平处理48h,G0/G1期细胞比例较对照组明显增高;经7.220μmol/L氨氯地平处理48h,MCF-7细胞中cyclinD1蛋白表达降低,p21和p53蛋白表达升高。结论氨氯地平对MCF-7细胞的增殖具有抑制作用,此作用与使细胞阻滞于G1期有关,其机制与调控细胞周期相关蛋白cyclinD1、p21和p53的表达有关。
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| 关 键 词： | 氨氯地平 乳腺癌 MCF-7细胞 细胞周期 周期蛋白 |
Effect of Amlodipine on Cell Cycle and Cyclin Expression of Human Breast Cancer MCF-7 Cells and Regulation Mechanism of The Effect |
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| HUANG Wen-jing,LI Wei-ping,SUN Wen-juan. Effect of Amlodipine on Cell Cycle and Cyclin Expression of Human Breast Cancer MCF-7 Cells and Regulation Mechanism of The Effect[J]. Chinese Journal of Bilogicals, 2010, 23(3) |
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| Authors: | HUANG Wen-jing LI Wei-ping SUN Wen-juan |
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| Affiliation: | HUANG Wen-jing,LI Wei-ping,SUN Wen-juan(Key Laboratory of Biochemistry , Molecular Pharmacollage of Chongqing,Department of Pharmacology,Pharmaceutical College,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective To investigate the effect of amlodipine on cycle and cyclin expression of human breast cancer MCF-7 cells as well as the regulation mechanism of the effect.Methods The MCF-7 cells at logarithmic growth phase were treated with amlodipine at various concentrations.The cell proliferation level was determined by MTT method,the cell cycle was measured by flow cytometry,and the expressions of cyclinD1,p21 and p53 were determined by immunocytochemical method.Results Amlodipine showed dose-and time-depend... |
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| Keywords: | Amlodipine Breast cancer MCF-7 cells Cell cycle Cyclin |
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