The essential function of Rrs1 in ribosome biogenesis is conserved in budding and fission yeasts |
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| Authors: | Kun Wan Haruka Kawara Tomoyuki Yamamoto Kazunori Kume Yukari Yabuki Tetsuya Goshima Kenji Kitamura Masaru Ueno Muneyoshi Kanai Dai Hirata Kouichi Funato Keiko Mizuta |
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| Affiliation: | 1. Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan;2. Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Japan;3. National Research Institute of Brewing, Higashi‐Hiroshima, Japan;4. Asahi‐shuzo Sake Brewing Co. Ltd, Niigata, Japan |
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| Abstract: | The Rrs1 protein plays an essential role in the biogenesis of 60S ribosomal subunits in budding yeast (Saccharomyces cerevisiae). Here, we examined whether the fission yeast (Schizosaccharomyces pombe) homologue of Rrs1 also plays a role in ribosome biogenesis. To this end, we constructed two temperature‐sensitive fission yeast strains, rrs1‐D14/22G and rrs1‐L51P, which had amino acid substitutions corresponding to those of the previously characterized budding yeast rrs1‐84 (D22/30G) and rrs1‐124 (L61P) strains, respectively. The fission yeast mutants exhibited severe defects in growth and 60S ribosomal subunit biogenesis at high temperatures. In addition, expression of the Rrs1 protein of fission yeast suppressed the growth defects of the budding yeast rrs1 mutants at high temperatures. Yeast two‐hybrid analyses revealed that the interactions of Rrs1 with the Rfp2 and Ebp2 proteins were conserved in budding and fission yeasts. These results suggest that the essential function of Rrs1 in ribosome biogenesis may be conserved in budding and fission yeasts. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | ribosome biogenesis fission yeast budding yeast Rrs1 |
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