| 灵芝发酵全粉中蛋白酶A抑制剂的提取及应用初探 |
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| 引用本文： | 田亚平,李屹松. 灵芝发酵全粉中蛋白酶A抑制剂的提取及应用初探[J]. 酿酒, 2003, 30(6): 90-92 |
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| 作者姓名： | 田亚平 李屹松 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室,江苏,无锡,214036 |
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| 摘 要： | 采用热水抽提、乙醇分级沉淀等步骤从灵芝发酵全粉中初步筛得一种粗蛋白酶A抑制剂；进一步采用supetdex-200和ACA44凝胶层析方法对其进行分离和组成分析，发现分子量38000蛋白质部分有较强抑制效果，一定条件下抑制率可达71％；应用实验中发现抑制剂的添加能明显提高纯生啤酒的泡沫稳定性。
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| 关 键 词： | 灵芝发酵全粉 蛋白酶A抑制剂 提取 应用 纯生啤酒 泡沫稳定性 |
| 文章编号： | 1002-8110(2003)06-0090-03 |
| 修稿时间： | 2003-08-16 |
Extraction and Primary Exploration on the Proteinase A Inhibitor from the Powder of Ganoderma lucidum by Fermentation |
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| TIAN Ya-ping,LI Yi-shong,LI Yu-na,GU Guo-xiang,ZHANG Ke-chang. Extraction and Primary Exploration on the Proteinase A Inhibitor from the Powder of Ganoderma lucidum by Fermentation[J]. Liquor Making, 2003, 30(6): 90-92 |
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| Authors: | TIAN Ya-ping LI Yi-shong LI Yu-na GU Guo-xiang ZHANG Ke-chang |
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| Abstract: | Through dipping in hot water and ethanol precipitation, a kind of proteinase A inhibitor can be selected from the powder of Ganoderma lucidum by submerged fermentation ; After separating and analyzing it by superdex 200 and ACA44 gel filtration, the molecular weight of protein which can stronger inhibit proteinase A is about 38KD, the inhibiting rate is 72% ; When we added this proteinase A inhibitor in the draft beer, the foam stability could be increased obviously. |
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| Keywords: | the powder of Ganoderma lucidum by fermentation proteinase A inhibitor draft beer foam stability |
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