自组装白蛋白共负载光敏剂及基因纳米递送系统的构建及初步评价

通过小分子十二烷基硫酸钠(SDS)调节牛血清白蛋白(BSA)疏水区域暴露,使白蛋白分子在不借助额外催化剂和交联剂的相对温和条件下自组装,合成了一种可共载光敏剂二氢卟吩e6(Ce6)及基因(CpG寡脱氧核苷酸)的白蛋白纳米载体。在其成功负载光敏剂Ce6后,用聚醚酰亚胺(PEI)对BSA纳米颗粒(BSA NP)表面进行了修饰,并通过调节合适的修饰比例得到高效负载基因的纳米体系。随后对纳米颗粒体系的形貌、尺寸分布、表面电荷、稳定性以及基因负载能力、产生活性氧能力进行了初步评估。结果表明合成得到的PEI修饰的白蛋白共负载Ce6及CpG(BSA-Ce6-PEI-CpG)的纳米颗粒尺寸分布均匀,微观形貌呈现光滑规整的圆球形,对CpG基因有较好的负载能力,并在光照下高效产生活性氧,且纳米体系能够在表面活性剂处理后依然保持结构稳定,可作为良好的光敏剂和基因递送载体。

Synthesis and Preliminary Evaluation of a BSA Co-delivery Nano System for Gene and Photosensitizer

The BSA co-delivery nano system was synthesized through a self-assembling method, by the well control of the BSA structure during heating process by SDS, the BSA nanocarrier was developed in a relatively mild condition without extra catalyst or cross-linker. Then the surface potential was modified after Ce6 photosensitizer was successfully loaded, and through rational regulation of surface potential by PEI decorating, a nano system with optimal efficiency for CpG (a nucleic acid) and Ce6 loading was organized. Subsequently the structure and morphology, size distribution, surface potential and stability were characterized, the results turned out that BSA-Ce6-PEI-CpG nano system owns well-dispersed, regular spherical nanoparticles at nanoscale. Moreover, the structure stability, effective encapsulation of CpG and ROS releasement upon irradiation proved it to be a promising co-carrier for gene and photosensitizer.