Facile development,characterization, and optimization of new metformin-loaded nanocarrier system for efficient colon cancer adjunct therapy |
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| Authors: | Kholoud Arafa Rehab N. Shamma Omaima N. El-Gazayerly |
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| Affiliation: | 1. Center for Aging and Associated Disease (CAAD), Zewail City of Science and Technology, Giza, Egypt;2. Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, Egypt |
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| Abstract: | Purpose: Metformin hydrochloride (MF) repurposing as adjuvant anticancer therapy for colorectal cancer (CRC) proved effective. Several studies attempted to develop MF-loaded nanoparticles (NPs), however the entrapment efficiency (EE%) was poor. Thus, the present study aimed at the facile development of a new series of chitosan (CS)-based semi-interpenetrating network (semi-IPN) NPs incorporating Pluronic® nanomicelles as nanocarriers for enhanced entrapment and sustained release of MF for efficient treatment of CRC.Methods: The NPs were prepared by ionic gelation and subsequently characterized using FTIR, DSC, TEM, and DLS. A full factorial design was also adopted to study the effect of various formulation variables on EE%, particle size, and zeta-potential of NPs.Results: NPs had a spherical shape and a mean particle size ranging between 135 and 220?nm. FTIR and DSC studies results were indicative of successful ionic gelation with the drug being dispersed in its amorphous form within CS-Pluronic® matrix. Maximum EE% reaching 57.00?±?12.90% was achieved using Pluronic®-123 based NPs. NPs exhibited a sustained release profile over 48?h. The MF-loaded NPs sensitized RKO CRC cells relative to drug alone.Conclusion: The reported results highlighted the novel utility of the developed NPs in the arena of colon cancer treatment. |
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| Keywords: | Metformin hydrochloride chitosan Pluronic® nanoparticles colorectal cancer |
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