龙须菜藻红蛋白对Hela细胞增殖抑制及其机制的研究

目的:探讨龙须菜藻红蛋白(PE)对人宫颈癌细胞Hela体外的抑制作用及其作用机制。方法:采用MTT法检测不同浓度的藻红蛋白对Hela细胞增殖抑制效果。流式细胞仪和Annexin V-FITC/PI双荧光染色法观察藻红蛋白对Hela细胞周期及细胞凋亡的影响。结果:PE可显著抑制Hela细胞的生长,并呈剂量-效应关系(p<0.01,r=0.84),剂量为20μg/ml,作用48h,其抑制率达70.88%,其48h的IC50值为4.12μg/ml。PE可阻滞Hela细胞从G2/M期进入S期,诱导细胞凋亡。结论:PE对Hela细胞有较强的抑制作用,其作用机制部分与诱导Hela细胞调亡有关。

Study on Inhibition Effects of Phycoerythrin from Gracilaria lemaneiformis on Hela Cells and Its Mechanism

Objective:To investigate the inhibition effects of phycoerythrin(PE)from Gracilaria lemaneiftheormis on growth of Hela cells and its mechanism.Method:The proliferation of cell lines of Hela in vitro was assayed by MTT method.Effects of PE on Hela cells cycle and apoptosis were observed by flow cytometry and Annexin V-FITC/PI fluorescence staining.Result:PE could obviously inhibit the proliferation of Hela cells with a fair dose-effect relationship(p<0.01,r =0.84).The inhibitory rate at 48 h could reach more than 70% and its half inhibitory concentration(IC50)was 4.12μg/ml.PE could prevent the transformation of Hela cell cycle from G2/M to S phase to cause apoptosis.Conclusion:PE shows potent inhibitory effects on growth of Hela cells,and the mechanism is related to inducing the apoptosis of Hela cells.