| 三角叶滨藜子叶愈伤组织诱导及分化的研究 |
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| 引用本文： | 周晓丽,刘广平. 三角叶滨藜子叶愈伤组织诱导及分化的研究[J]. 辽东学院学报(自然科学版), 2004, 11(1): 20-21,17 |
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| 作者姓名： | 周晓丽 刘广平 |
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| 作者单位： | 辽东学院,师资部,辽宁,丹东,118005;辽宁省经济林研究所,辽宁,大连,116038 |
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| 摘 要： | 以经过灭菌处理的三角叶滨藜种子为材料,以MS和1/2MS+G30或15两个浓度组合的培养基为基本培养基,附加不同浓度的生长素和细胞分裂素,对三角叶滨藜子叶愈伤组织的诱导和分化进行了研究。获得三角叶滨藜幼苗旺盛生长的培养基为1/2MS加G15;在附加不同浓度BA、IAA、IBA、NAA、2,4-D的MS培养基上,筛选出诱导子叶愈伤组织的最适培养基是MS+BA2/L+2,4-D1/L。把愈伤组织接种到附加不同浓度IAA、GA的MS+BAI/L培养基上进行分化培养,筛选出愈伤组织分化的理想培养基是MS+BAI/L+IAAO.2/L+GA2/L。
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| 关 键 词： | 三角叶滨藜 子叶 愈伤组织 分化 无性系 |
| 文章编号： | 1008-2174(2003)04-0020-03 |
| 修稿时间： | 2003-10-12 |
Studied on the Induction and Differentiation of Tissue Culture From Cotyledon of Atriplex Triangularis |
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| ZHOU Xiao-li,LIU Guang-ping Liaodong Institute,Dandong ,China,Liaoning Resenrch Institute of Economic Forest,Dalian ,China. Studied on the Induction and Differentiation of Tissue Culture From Cotyledon of Atriplex Triangularis[J]. Journal of Liaodong University(Natural Sciences), 2004, 11(1): 20-21,17 |
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| Authors: | ZHOU Xiao-li LIU Guang-ping Liaodong Institute Dandong China Liaoning Resenrch Institute of Economic Forest Dalian China |
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| Affiliation: | ZHOU Xiao-li,LIU Guang-ping Liaodong Institute,Dandong 118005,China,Liaoning Resenrch Institute of Economic Forest,Dalian 116038,China |
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| Abstract: | General mediums are MS and 1/2MS, adding two kinds of quantity G15 g/L and G30g/L. After antidotingthe seeds of Atriplex triangularis are used as explants. The ideal medium for Atriplex triangularis healthygrowth is 1/2MS+G15g/L; then based on that condition different hormone concentration of BA、IBA、NAA、and 2,4-D are added to the MS medium. The medium that suit for the induction of Atriplex triangularis's cotyledonis MS+BA2/L+2, 4-D1/L. The callus were transferred to the medium for differentiation, the ideal medium isMS+BA1/L+IAAGA2. |
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| Keywords: | atriplex triangularis cotyledon callus differentiation asexual systerm |
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