| 反相液相色谱法测定(反)-4-环已基-L-脯氨酸和(反)-4-苯基-L-脯氨酸 |
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| 引用本文： | 施介华,李婷,彭丽,周虎,ZHOU Hu. 反相液相色谱法测定(反)-4-环已基-L-脯氨酸和(反)-4-苯基-L-脯氨酸[J]. 浙江工业大学学报, 2010, 38(1) |
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| 作者姓名： | 施介华 李婷 彭丽 周虎 ZHOU Hu |
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| 作者单位： | 1. 浙江工业大学,药学院,浙江,杭州310032
2. 浙江华海药业股份有限公司,浙江,临海317024 |
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| 基金项目： | 浙江省科技厅资助项目(2006C23013) |
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| 摘 要： | 采用反相高效液相色谱法分离和测定(反)-4-环已基-L-脯氨酸和(反)-4-苯基-L-脯氨酸.在选定的色谱条件下,(反)-4-环已基-L-脯氨酸、(反)-4-苯基-L-脯氨酸和杂质之间具有较好的分离效果.该方法具有操作简单、快速、准确度和精密度较好,对同一(反)-4-环已基-L-脯氨酸试样多次测定,(反)-4-环已基-L-脯氨酸的日内、日间相对标准偏差(RSD)分别1.55%和0.09%;(反)-4-苯基-L-脯氨酸的日内、日间相对标准偏差(RSD)分别1.94%和1.79%.(反)-4-环已基-L-脯氨酸和(反)-4-苯基-L-脯氨酸的回收率分别为99.80%~100.40%和97.28%~100.00%.
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| 关 键 词： | (反)-4-环已基-L-脯氨酸 (反)-4-苯基-L-脯氨酸 高效液相色谱 |
Determination of (trans)-4-cyclohexyl-L-proline and (trans)-4-phenyl-L-proline by high-performance liquid chromatography |
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| SHI Jie-hua,LI Ting,PENG Li,YAN Wei,ZHOU Hu. Determination of (trans)-4-cyclohexyl-L-proline and (trans)-4-phenyl-L-proline by high-performance liquid chromatography[J]. Journal of Zhejiang University of Technology, 2010, 38(1) |
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| Authors: | SHI Jie-hua LI Ting PENG Li YAN Wei ZHOU Hu |
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| Affiliation: | SHI Jie-hua1,LI Ting1,PENG Li1,YAN Wei1,ZHOU Hu2(1.College of Pharmaceutical Sciences,Zhejiang University of Technology,Hangzhou 310032,China,2.Zhejiang Huahai Pharmaceutical Co.,Ltd.,Linhai 317024,China) |
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| Abstract: | (Trans)-4-cyclohexyl-L-proline and(trans)-4-phenyl-L-proline has been separated and determined by reversed-phase HPLC.The separation efficiency of(trans)-4-cyclohexyl-L-proline,(trans)-4-phenyl-L-proline and other impurities was high under the selected conditions of the chromatographic operation.The accuracy and precision of this method were satisfied.The repeated tests of(trans)-4-cyclohexyl-L-proline sample showed that RSD of inter-day and intra-day for(trans)-4-cyclohexyl-L-proline were 1.55% and 0.09% r... |
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| Keywords: | (trans)-4-cyclohexyl-L-proline (trans)-4-phenyl-L-proline high-performance liquid chromatography |
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