Kinetic study of an enzyme-catalysed reaction in the presence of novel irreversible-type inhibitors that react with the product of enzymatic catalysis |
| |
Authors: | MJ Navarro-Lozano E Valero R Varon F Garcia-Carmona |
| |
Affiliation: | Departmento de Química-Física, Escuela Universitaria Politécnica de Albacete, Universidad de Castilla-La Mancha, Spain. |
| |
Abstract: | In the present paper a kinetic study is made of the behaviour of a Michaelis-Menten enzyme-catalysed reaction in the presence of irreversible inhibitors rendered unstable in the medium by their reaction with the product of enzymatic catalysis. A general mechanism involving competitive, non-competitive, uncompetitive and mixed irreversible inhibition with one or two steps has been analysed. The differential equation that describes the kinetics of the reaction is non-linear and computer simulations of its dynamic behaviour are presented. The results obtained show that the systems studied here present kinetic co-operativity for a target enzyme that follows the simple Michaelis-Menten mechanism in its action on the substrate, except in the case of an uncompetitive-type inhibitor. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|