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SPR生物传感技术的研究进展与应用
引用本文:杨雨曦,孙雅婷,杨梦朝,金伟,牟颖.SPR生物传感技术的研究进展与应用[J].生命科学仪器,2014(5):7-16.
作者姓名:杨雨曦  孙雅婷  杨梦朝  金伟  牟颖
作者单位:浙江大学控制科学与工程学系控制技术国家重点实验室浙江大学分析仪器研究中心,杭州310058
基金项目:国家自然科学基金(31270907); 国家科技部“十一五”支撑计划项目课题(2006BAK03A09)资助
摘    要:由于具有实时、免标记、高灵敏度等优点,表面等离体子共振(surface plasmon resonance,SPR)技术被广泛应用于生物分子相互作用的研究分析。在SPR能够检测的分子量范围内,基本所有的具有特异性反应的生物分子都可以被SPR生物传感器检测。因此,SPR技术越来越展示出其重要的应用价值,成为近几年来研究的热点。本文重点从原理、技术改进以及应用等三个角度对SPR技术进行阐述,并从技术改进方面重点介绍了SPR技术的两个发展:表面等离子体子共振成像(SPRi)技术与局域表面等离子体子技术(LSPR)。

关 键 词:表面等离子体子共振(SPR)  表面等离子体子共振成像(SPRi)  局域表面等离子体子共振(LSPR)

Advances and Applications of Surface Plasmon Resonance Biosensing
Yang Yuxi,Sun Yating,Yang Mengchao,Jin Wei,Mu Ying.Advances and Applications of Surface Plasmon Resonance Biosensing[J].Life Science Instruments,2014(5):7-16.
Authors:Yang Yuxi  Sun Yating  Yang Mengchao  Jin Wei  Mu Ying
Affiliation:(Research Center for Analytical Instrumentation, State Key Laboratory of Industrial Control Technology, Department of Control Science and engineering, Zhejiang University, Hangzhou, China)
Abstract:With the superiorities as real-time, lable-free and precise, surface plasmon resonance(SPR) has been widely used in analysis of the interactions of biomolecules. Almost all the biomolecules with specific reactions can be detected by SPR biosensor within its limits of molecular weight. As a result, the value of applications of SPR has been revealed continually, which makes this technology become the research hotspot in recent years. We expounded SPR from three aspects, which are principle, the newest advances and applications. We also introduced local surface plasmon resonance(LSPR) and surface plasmon resonance imaging(SPRi) as two aspects of development of SPR.
Keywords:Surface plasmon resonance(SPR)  Local surface plasmon resonance(LSPR)  Surface plasmon resonance imaging(SPRi)
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