| 转基因玉米 BT11 品系环介导等温扩增(LAMP)检测方法的建立 |
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| 引用本文： | 王清华,徐君怡,曹冬梅,刘 宇,曹际娟. 转基因玉米 BT11 品系环介导等温扩增(LAMP)检测方法的建立[J]. 食品安全质量检测学报, 2013, 4(3): 868-872 |
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| 作者姓名： | 王清华 徐君怡 曹冬梅 刘 宇 曹际娟 |
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| 作者单位： | 大连工业大学生物工程学院,辽宁出入境检验检疫局,辽宁出入境检验检疫局,辽宁出入境检验检疫局,辽宁出入境检验检疫局 |
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| 基金项目： | 国家转基因重大专项(2013ZX08012-001) |
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| 摘 要： | 目的 建立转基因玉米 BT11 品系的环介导等温扩增(LAMP)检测方法。方法 根据转基因玉米 BT11品系特有序列(AY123624.1 和 AY629236)设计引物, 优化建立 LAMP 反应体系, 对该体系进行特异性、 灵敏度、稳定性分析。结果 本研究设计的引物具有很好的特异性, 可特异性检测出转基因玉米 BT11 品系; 检测灵敏度可达 0.5%; 经精密度实验分析, 该方法的稳定性良好。结论 LAMP 方法检测转基因玉米品系 BT11 具有特异性高、稳定性好、快速灵敏、过程可视等优点, 具有广阔的应用前景。
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| 关 键 词： | 转基因玉米 BT11 环介导等温扩增(LAMP) 品系鉴定检测 |
| 收稿时间： | 2013-04-17 |
| 修稿时间： | 2013-05-31 |
Establishing a loop-mediated isothermal amplification(LAMP)detection method for genetically modified maize BT11 |
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| WANG Qing-Hu,XU Jun-Yi,CAO Dong-Mei,LIU Yu and CAO Ji-Juan. Establishing a loop-mediated isothermal amplification(LAMP)detection method for genetically modified maize BT11[J]. Journal of Food Safety & Quality, 2013, 4(3): 868-872 |
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| Authors: | WANG Qing-Hu XU Jun-Yi CAO Dong-Mei LIU Yu CAO Ji-Juan |
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| Affiliation: | School of Biological Engineering, Dalian Polytechnic University,Liaoning Entry-Exit Inspection and Quarantine Bureau,Liaoning Entry-Exit Inspection and Quarantine Bureau,Liaoning Entry-Exit Inspection and Quarantine Bureau,Liaoning Entry-Exit Inspection and Quarantine Bureau |
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| Abstract: | Objective To establish a loop-mediated isothermal amplification(LAMP)detection method for BT11. Methods The primers were designed according to the specific sequence(AY123624.1, AY629236)of BT11, and then the LAMP reaction system was established. The specificity, sensitivity and stability of the sys-tem were analyzed. Results The primers in this research specifically detected the BT 11. The detection sensi-tivity was up to 0.5%, and the precision of the experimental analysis was proved to be of high stability. Con-clusion The results showed that LAMP was a specific, stable, rapid, sensitive and visible way for the detec-tion of BT 11, and it would have broad prospects for application. |
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| Keywords: | genetically modified maize BT11 LAMP identification of lines |
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