Matrix Addressing of an Electronic Surface Switch Based on a Conjugated Polyelectrolyte for Cell Sorting |
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Authors: | Kristin M Persson Susanna Lönnqvist Klas Tybrandt Roger Gabrielsson David Nilsson Gunnar Kratz Magnus Berggren |
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Affiliation: | 1. Department of Science and Technology, Link?ping University, Norrk?ping, Sweden;2. Department of Clinical and Experimental Medicine, Link?ping University, Link?ping, Sweden;3. Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Zurich, Switzerland;4. Department of Physics, Chemistry and Biology, Link?ping University, Link?ping, Sweden;5. Department of Printed Electronics, Acreo Swedish ICT AB, Norrk?ping, Sweden;6. Department of Hand and Plastic Surgery, Link?ping University, Link?ping, Sweden |
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Abstract: | Spatial control of cell detachment is potentially of great interest when selecting cells for clonal expansion and in order to obtain a homogeneous starting population of cells aimed for tissue engineering purposes. Here, selective detachment and cell sorting of human primary keratinocytes and fibroblasts is achieved using thin films of a conjugated polymer. Upon electrochemical oxidation, the polymer film swells, cracks, and finally detaches taking cells cultured on top along with it. The polymer can be patterned using standard photolithography to fabricate a cross‐point matrix with polymer pixels that can be individually addressed and thus detached. Detachment occurs above a well‐defined threshold of +0.7 V versus Ag/AgCl, allowing the use of a relatively simple and easily manufactured passive matrix‐addressing configuration, based on a resistor network, to control the cell‐sorting device. |
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Keywords: | biomedical applications conjugated polymers organic electronics photolithography tissue engineering |
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