| 棉织物上SESA活化法固定化脂肪酶工艺的研究 |
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| 引用本文： | 彭立凤,刘新喜. 棉织物上SESA活化法固定化脂肪酶工艺的研究[J]. 食品工业科技, 2001, 0(2): 30-32 |
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| 作者姓名： | 彭立凤 刘新喜 |
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| 作者单位： | 1. 河北师范大学化学系,
2. 河北师范大学学报编辑部, |
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| 基金项目： | 河北省自然科学基金资助项目(299211) |
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| 摘 要： | 研究了对-β-硫酸酯乙砜基苯胺活化法在棉织物上固定化脂肪酶的工艺条件，并考察了固定化酶的最适作用温度和pH及间歇操作稳定性。最佳固定化条件为醚化pH为10.0，偶联pH为7.0，脂肪酶的浓度为6mg/ml，偶联时间为12h，所得固定化脂肪酶的最大活力为35U/g(棉)·min。固定化酶的最适作用温度为35℃，最适pH为8.0，半衰期为6d。
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| 关 键 词： | 脂肪酶固定化棉织物对-β-硫酸酯乙砜基苯胺(SESA) |
Study on immobilization of lipase onto cotton fiber activated by SESA |
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| Peng Lifeng et al.. Study on immobilization of lipase onto cotton fiber activated by SESA[J]. Science and Technology of Food Industry, 2001, 0(2): 30-32 |
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| Authors: | Peng Lifeng et al. |
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| Affiliation: | Peng Lifeng et al. |
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| Abstract: | The conditions of the lipase immobilization onto cotton fiber activated by p-β-sulfuric acid ester ethyl sulfone aniline (SESA) have been studied. The optimal temperature and pH of the immobilized lipase were investigated, and the operating stability of the immobilized lipase was studied too. The results showed that the optimal conditions for the immobilization were that the pH of the reaction for the cotton fiber with SESA was 10.0,the linking pH of lipase to fiber was 7.0,the linking time was 12h,and the concentration of lipase was 6mg· mL- 1 .Then 35 U· g(fiber) - 1 · min- 1 activity of immobilized lipase was obtained. The optimal temperature and pH of the immobilized lipase were 35 ℃ and 8.0 respectively, and its half life was 6 days . |
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