Phospholipase C catalyzed formation of sphingomyelin-14C from lecithin and N-(14C)-oleoyl-sphingosine |
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| Authors: | Julian N Kanfer Christine H Spievlogel |
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| Affiliation: | 1. Eunice Kennedy Shriver Center at the W.E. Fernald State School, 02154, Waltham, Massachusetts
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| Abstract: | Commercial preparations of Clostreidium perfringens were incubated with phosphatidyl choline and N-1-(-14C) oleoylsphingosine. A radioactive product was formed which cochromatogramed with spingomyelin standard in three different solvent systems. Several other phospholipases and phosphatases were unable to catalyze this reaction. Neither choline, phosphoryl choline, cytidine diphosphate choline nor p-nitrophenyl phosphoryl choline were acitve donors. Sphingomyelin was only slightly active as a phosphoryl choline donor. |
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