| 镍与绿茶EGCG对舌鳞癌细胞毒性的影响 |
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| 引用本文： | 罗真兰,王海波,苏俭生.镍与绿茶EGCG对舌鳞癌细胞毒性的影响[J].无机材料学报,2012(2):179-184. |
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| 作者姓名： | 罗真兰 王海波 苏俭生 |
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| 作者单位： | 同济大学口腔医学院 |
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| 基金项目： | 国家自然科学基金(30840031,30970726)~~ |
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| 摘 要： | Ni2+是镍基牙科铸造合金中析出的主要金属离子,是人体的致癌物质.绿茶的主要活性成分表没食子儿茶素没食子酸酯(EGCG)对包括口腔癌在内的人体癌有化学预防作用.本研究采用LC/MS法分析EGCG与Ni2+间的相互作用.MTT法检测EGCG、Ni2+及其相互作用对舌鳞癌细胞生长的影响.LC/MS分析显示,Ni2+与EGCG同时加入时有EGCG-2Ni2+]和EGCG-Ni2+]形成.Ni2+和EGCG在一定范围内对舌鳞癌细胞生长的抑制呈时间及浓度依赖性.EGCG与Ni2+共同作用后可明显增强对舌鳞癌细胞的抑制作用,且抑制作用与它们的浓度、加入的顺序及Ni2+与EGCG的比例有关.
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| 关 键 词： | 牙科铸造合金 绿茶 Ni2+ EGCG 舌鳞癌细胞 |
Effect of Interaction of Ni2+ and Green Tea Epigallocatechin Gallate on Tongue Carcinoma Cells |
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| LUO Zhen-Lan,WANG Hai-Bo,SU Jian-Sheng.Effect of Interaction of Ni2+ and Green Tea Epigallocatechin Gallate on Tongue Carcinoma Cells[J].Journal of Inorganic Materials,2012(2):179-184. |
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| Authors: | LUO Zhen-Lan WANG Hai-Bo SU Jian-Sheng |
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| Affiliation: | (School of Stomatology,Tongji University,Shanghai 200072,China) |
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| Abstract: | Ni2+ is the major ion release from nickel-based dental casting alloy and carcinogens.The major polyphenolic constituent present in green tea,(-)epigallocatechin-3-gallate(EGCG),shows chemoprevention on human cancer.The aim of this study was to determine the interaction between EGCG and Ni2+ and the biological effects on tongue squamous cell carcinoma.The interaction of EGCG and Ni2+ was analyzed by LC/MS.The cell viability was assessed by the MTT reduction assay.The results of LC/MS indicated that EGCG-2Ni2+] and EGCG-Ni2+] formed when EGCG and Ni2+ were added simultaneously.Ni2+ and EGCG both inhibited the proliferation of tongue carcinoma cells in a time-and dose-dependent manner.The cytotoxicity against tongue carcinoma cells was increased when EGCG and Ni2+ cotreated,and the inhibition effects were extremely dependent on the concentrations of EGCG and Ni2+,addition order and the ratio of Ni2+] to EGCG]. |
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| Keywords: | dental casting alloy green tea Ni2+ EGCG: tongue carcinoma cells |
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