| 木糖发酵产2，3-丁二醇培养基的优化 |
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| 引用本文： | 肖冬光,韩宁宁,张翠英,郭学武.木糖发酵产2，3-丁二醇培养基的优化[J].酿酒科技,2010(4):26-30. |
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| 作者姓名： | 肖冬光 韩宁宁 张翠英 郭学武 |
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| 作者单位： | 工业微生物教育部重点实验室,天津市工业微生物重点实验室,天津科技大学生物工程学院,天津,300457 |
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| 基金项目： | 天津科技大学科学研究基金项目(20090401) |
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| 摘 要： | 以2,3-丁二醇得率作为考察指标,运用单因素试验和4因素3水平响应面实验设计方法,研究木糖、氮源、无机盐和金属离子对2,3-丁二醇得率的影响。结果表明,培养基的最佳浓度组合为:木糖为83.4 g/L、酵母粉为20.3 g/L、KH2PO4为10 g/L、K2HPO4为7.2 g/L(、NH4)2SO4 2 g/L、柠檬酸钠4 g/L、MgSO4.7H2O 0.05 g/L、MnSO4.7H2O为0.005 g/L、ZnSO4.7H2O 0.01 g/L、FeSO4.7H2O0.005 g/L、CaCl2 0.057 g/L。利用优化后的培养基进行发酵,2,3-丁二醇得率达0.348 g/g,与初始条件相比,提高了16%;实验结果与模型预测值拟合一致。
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| 关 键 词： | 2 3-丁二醇 木糖 发酵培养基 响应面法 |
Optimization of Xylose-based Fermentation Medium for the Production of 2,3-Butanediol by Response Surface Method |
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| XIAO Dong-guang,HAN Ning-ning,ZHANG Cui-ying,GUO Xue-wu.Optimization of Xylose-based Fermentation Medium for the Production of 2,3-Butanediol by Response Surface Method[J].Liquor-making Science & Technology,2010(4):26-30. |
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| Authors: | XIAO Dong-guang HAN Ning-ning ZHANG Cui-ying GUO Xue-wu |
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| Affiliation: | Key Lab of Industrial Microbiology/a>;Ministry of Education/a>;Tianjin Industrial Microbiology Key Lab/a>;College of Biotechnology/a>;Tianjin University of Science and Technology/a>;Tianjin 300457/a>;China |
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| Abstract: | The effects of xylose,nitrogen source,inorganic salt and metal ions on 2,3-butanediol yield were investigated by single factor design and response surface method(four factors and three levels).The results showed that the optimum compositions of the fermentation medium were as follows: xylose 83.4 g/L,yeast powder 20.3 g/L,KH2PO4 10 g/L,K2HPO4 7.2 g/L,(NH4)2SO4 2 g/L,sodiumcitrate 4 g/L,MgSO4.7H2O 0.05 g/L,MnSO4.7H2O 0.005 g/L,ZnSO4.7H2O 0.01 g/L,FeSO4.7H2O 0.005 g/L,and CaCl2 0.057 g/L.Under the above condi... |
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| Keywords: | 2 3-butanediol xylose fermentation medium response surface methodology |
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