鸡腿菇菌丝深层培养富硒的研究

通过正交试验,从4株鸡腿菇菌株中筛选出1株具有较高生物量和较强富硒能力的鸡腿菇菌株CcⅢ,并探讨了其耐硒和富硒特性。结果表明,CcⅢ菌株具有一定的耐硒能力和很强的富硒能力,其固态培养最大耐硒浓度为10μg/mL,液态培养最大耐硒浓度为4μg/mL。在硒浓度2μg/mL时,菌丝体生物量为4.11 g/L,富硒量246.74μg/g,富硒率为50.57%,有机化程度高达92.95%。

A Study on the Se-Accumulation of Coprinus comatus Mycelia in Submerged Culture

In this paper,the mycelia biomass and the ability of selenium (Se) accumulation of four strains of Coprinus comatus were firstly investigated.Although the strain of Coprinus comatusⅢdid not show the highest production of mycelia biomass,it showed the highest ability of Se accumulation according to the or- thogonal experimental design.Thus Coprinus comatusⅢwas selected to test the characters of Se tolerance and accumulation.The result showed that the production of mycelia biomass and Se-accumulation was 4.11g/L and 246.74μg/g espectively when the concentration of Se is 2μg/mL while the ratio of Se accumu- lation and organic Se was 50.57% and 92.95% respectively under the same condition.This result provides a new way for the Chinese people to resolve the shortage of Se uptake.