乳酸菌对自由基清除能力的研究

研究了52株乳酸菌的细胞和无细胞提取物清除DPPH自由基的能力,从中筛选出了3株对DPPH自由基清除率较高的乳酸菌,并对它们清除超氧阴离子自由基、羟自由基的能力及其还原能力做了进一步研究。结果表明,ZS-2的无细胞提取物对DPPH的清除率最高(46.53%),对羟自由基具有较高的清除率(48.72%),但对超氧阴离子自由基没有清除作用;而FS-3和BCX-9细胞对羟自由基的清除率均高于它们的无细胞提取物,同时也具有较强的清除超氧阴离子的能力。其中,FS-3无细胞提取物对超氧阴离子自由基的清除率最高,3株菌均有一定的还原能力。研究表明,乳酸菌的细胞和无细胞提取物在体外实验中对各种自由基具有不同的清除能力。

Study on the scavenging ability of lactic acid bacteria on free radicals

The scavenging ability of 52 strains on DPPH free radicals were introduced in this paper and screened out 3 strains which have higher scavenging ability on DPPH radicals,and studied about their scavenging abilities on superoxide anion radicals,hydroxyl radicals and reducing activity.According to the data,the cell-free extracts of ZS-2 showed the highest ability to scavenge DPPH free radicals(46.53%) and higher scavenging ability on hydroxyl radicals.The intact cell and cell-free extracts of ZS-2 on superoxide anion radicals were not detected.The intact cell of FS-3 and BCX-9 on hydroxyl radicals showed higher scavenging effect than the cell-free extracts and better scavenging ability on superoxide anion.The cell-free extracts of FS-3 showed the highest scavenging effect on superoxide anion radicals.Three strains showed the reducing activity.The study demonstrated that the intact cell and the cell-free extracts of lactic acid bacteria have different scavenging ability in vitro.