| 硅胶柱色谱和反相高效液相色谱结合分离制备亚油酸海藻糖单酯 |
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| 引用本文： | 孙月娥,夏文水. 硅胶柱色谱和反相高效液相色谱结合分离制备亚油酸海藻糖单酯[J]. 食品与发酵工业, 2008, 34(12) |
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| 作者姓名： | 孙月娥 夏文水 |
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| 作者单位： | 江南大学食品科学与技术国家重点实验室,江苏,无锡,214122 |
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| 摘 要： | 采用固定化脂肪酶作生物催化剂在叔丁醇体系中成功的合成了亚油酸海藻糖单酯。通过硅胶柱色谱和反相高效液相色谱(RPLC)相结合的方法,得到了高纯度的亚油酸海藻糖单酯样品。将洗脱液经过旋转蒸发浓缩、冷冻干燥后,得到白色晶体,用电喷雾电离质谱(ESI-MS)和核磁共振氢谱(1HNMR)以及碳谱(13CNMR)对目标产物进行了确认,鉴定为亚油酸海藻糖单酯。该法具有制备量大和分离效率高的特点。
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| 关 键 词： | 亚油酸海藻糖单酯 固定化脂肪酶 高效液相色谱 硅胶柱色谱 核磁共振 |
Separation of Linoleoyl Trehalose by HPLC Combined With Silica Gel Column Chromatography |
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| Sun Yuee,Xia Wenshui. Separation of Linoleoyl Trehalose by HPLC Combined With Silica Gel Column Chromatography[J]. Food and Fermentation Industries, 2008, 34(12) |
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| Authors: | Sun Yuee Xia Wenshui |
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| Abstract: | Linoleoyl trehalose was synthesized by enzymatic esterification in tert-butanol solvent system.Reverse High performance liquid chromatography(rHPLC)combined with silica gel column chromatography was developed for the separation of the linoleoyl trehalose.The eluted solution was evaporated and vacuum freeze-dried to obtain linoleoyl trehalose.Electrospray ionization mass spectrometry(MS),1H nuclear magnetic resonance(1H NMR)and 13C nuclear magnetic resonance(13C NMR)were applied for structure conformation.The results showed that the sample identical to linoleoyl trehalose.The method was of high in capacity and efficiency in separation. |
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| Keywords: | linoleoyl trehalose immobiliized lipase high performance liquid chromatography(HPLC) silica gel column chromatography nuclear magnetic resonance |
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