樱桃籽中抗氧化物质的超声提取工艺及其抗氧化活性

以DPPH自由基清除法检测提取物的抗氧化能力,采用Box-Behnken试验设计结合响应面分析法确定超声提取樱桃籽中抗氧化物质的最佳工艺条件.结果表明,超声提取樱桃籽清除DPPH自由基物质的优化工艺条件为超声功率500W、液料体积质量比30 mL/g、乙醇体积分数40％、提取温度50℃、提取时间5 min.在最佳提取条件下提取的原液,其总黄酮质量分数为(11.13±0.48) mg/g,具有较强的还原能力和显著的清除DPPH及羟基自由基的能力.

Optimized Extraction Conditions and Antioxidant Activities of Ultrasonic-Extracted Antioxidant Components from Cherry Seed

In this study,with the DPPH radical scavenging as the index of antioxidant activity,the Box-Behnken design combined with response surface methodology(RSM) was used to optimize the ultrasonic extraction conditions of the antioxidant components from cherry seed.The optimum extraction processing parameters were determined as follows: ultrasonic power 500 W,liquid/material ratio(mL/g) 30:1,ethanol concentration 40%(V/V),extraction temperature 50℃,and extraction time 5 min.Under the optimum conditions,the total flavonoids content is(11.13 ± 0.48) mg/g of the extracts,and the extracts exhibited significant the antioxidant activities such as DPPH free radical and hydroxyl free radical scavenging effects,and reducing power.