| Parameter Optimization for Enhancement of Ethanol Yield by Atmospheric Pressure DBD-TreatedSaccharomyces cerevisiae简 |
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| 引用本文： | 董晓宇,袁玉莲,唐乾,窦少华,底兰波,张秀玲.Parameter Optimization for Enhancement of Ethanol Yield by Atmospheric Pressure DBD-TreatedSaccharomyces cerevisiae简[J].等离子体科学和技术,2014(1):73-78. |
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| 作者姓名： | 董晓宇 袁玉莲 唐乾 窦少华 底兰波 张秀玲 |
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| 基金项目： | supported by National Natural Science Foundation of China(Nos.21246012,21306015);Liaoning Provincial Public Welfare Research Foundation for Science Career of China(No.2011005001);Liaoning Provincial Natural Science Foundation of China(No.201205531);Scientific Research Fund of Liaoning Provincial Education Department(No.L2011220) |
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| 摘 要： | In this study, Saccharomyces cerevisiae （S. cerevisiae） was exposed to dielectric barrier discharge plasma （DBD） to improve its ethanol production capacity during fermenta- tion. Response surface methodology （RSM） was used to optimize the discharge-associated pa- rameters of DBD for the purpose of maximizing the ethanol yield achieved by DBD-treated S. cerevisiae. According to single factor experiments, a mathematical model was established using Box-Behnken central composite experiment design, with plasma exposure time, power supply volt- age, and exposed-sample volume as impact factors and ethanol yield as the response. This was followed by response surface analysis. Optimal experimental parameters for plasma discharge- induced enhancement in ethanol yield were plasma exposure time of 1 rain, power voltage of 26 V, and an exposed sample volume of 9 mL. Under these conditions, the resulting yield of ethanol was 0.48 g/g, representing an increase of 33% over control.
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| 关 键 词： | 酿酒酵母 参数优化 DBD 乙醇 产量 响应面分析法 常压 放电等离子体 |
Parameter Optimization for Enhancement of Ethanol Yield by Atmospheric Pressure DBD-Treated Saccharomyces cerevisiae |
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| Abstract: | In this study, Saccharomyces cerevisiae(S. cerevisiae) was exposed to dielectric barrier discharge plasma(DBD) to improve its ethanol production capacity during fermentation. Response surface methodology(RSM) was used to optimize the discharge-associated parameters of DBD for the purpose of maximizing the ethanol yield achieved by DBD-treated S. cerevisiae. According to single factor experiments, a mathematical model was established using Box-Behnken central composite experiment design, with plasma exposure time, power supply voltage, and exposed-sample volume as impact factors and ethanol yield as the response. This was followed by response surface analysis. Optimal experimental parameters for plasma dischargeinduced enhancement in ethanol yield were plasma exposure time of 1 min, power voltage of 26 V, and an exposed sample volume of 9 mL. Under these conditions, the resulting yield of ethanol was 0.48 g/g, representing an increase of 33% over control. |
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| Keywords: | dielectric barrier discharge(DBD) plasma at atmospheric pressure ethanol yield response surface methodology(RSM) Saccharomyces cerevisiae |
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