Association of phosphoinositide-specific phospholipase Cgamma1 with elements of cytoskeleton in A-431 cells |
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Authors: | SV Chupreta AL Evdonin DV Tvorogov NV Tsupkina NN Nikol'nski? ND Medvedeva |
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Affiliation: | Institute of Cytology, Russian Academy of Sciences, St. Petersburg. |
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Abstract: | It is shown that phosphoinositide-specific phospholipase C gamma 1 (PLC gamma 1), a substrate of growth factor receptors, is associated with cytoskeleton in A-431 cells. PLC gamma 1 is co-localized only with the cortical actin but not with actin stress fibers. Since EGF receptor is also co-localized with the cortical actin it is concluded that PLC gamma 1 co-localized with actin is mediated by the EGF receptor. After the treatment with cytochalasin B PLC gamma 1 is co-localized with actin aggregates and cytoskeleton elements other than actin. Using double immunofluorescence PLC gamma 1 is shown to be associated with cytokeratin intermediate filaments. The cross-talking of different cytoskeleton elements and their participation in cell signaling is discussed. |
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