Evaluation of the mechanical properties,in vitro biodegradability and cytocompatibility of natural chitosan/hydroxyapatite/nano-Fe3O4 composite |
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Authors: | Fatemeh Heidari Mehdi Razavi Mohammad E. Bahrololoom Mostafa Yazdimamaghani Mohammadreza Tahriri Hari Kotturi Lobat Tayebi |
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Affiliation: | 1. Department of Materials Science and Engineering, School of Engineering, Yasouj University, Yasuj 75918-74934, Iran;2. Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA 94304, USA;3. Department of Materials Science and Engineering, School of Engineering, Shiraz University, Shiraz, Iran;4. Helmerich Advanced Technology Research Center, Oklahoma State University, Tulsa, OK 74106, USA;5. Marquette University School of Dentistry, Milwaukee, WI 53233, USA;6. Department of Biology, University of Central Oklahoma, Edmond, OK 73034, USA;g. Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK |
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Abstract: | The main goal of this research was the preparation and evaluation of the mechanical properties, in vitro biodegradability and cytocompatibility, of natural chitosan/hydroxyapatite/nano magnetite (nano-Fe3O4) composite. Different ratios of these components were investigated, including chitosan/hydroxyapatite: 4/4 (S1), chitosan/hydroxyapatite: 4/6 (S2), and chitosan/hydroxyapatite: 6/4 (S3). Mechanical properties of fabricated composites were examined using bending and compression tests before immersion, and after 2 and 9 weeks of immersion in the Ringer's solution. Scanning electron microscope (SEM) was employed for observing the bending fracture surface and analyzing the degradation morphology. Human mesenchymal stem cells (hMSC) were also cultured on the samples in order to assess the cytocompatibility. The obtained results revealed that S1 had the highest bending strength before immersion, while S3 had the highest bending strength after 9 weeks immersion. Compressive strength of S2 was greater than that of S1 and S3 not only before immersion, but also after 9 weeks immersion. Although the bio-minerals were deposited on the surface of all samples during the immersion in Ringer's solution, S2 appeared to have the highest quantity of bio-minerals. According to the weight loss percentage (ΔW(%)), the biodegradation resistance of S1 was the lowest. Finally, the cytocompatibility of S1 was greater than that of S2 and S3. |
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Keywords: | Chitosan Hydroxyapatite Magnetite Nanocomposite Mechanical properties Biodegradability |
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