Piroxicam benzoate--synthesis,HPLC determination,and hydrolysis |
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Authors: | Boneschans Barend Wessels Anita van Staden Johan Zovko Marijana Zorc Branka Bergh Jacobus |
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Affiliation: | a Center for Quality Assurance of Medicines, Potchefstroom University, Potchefstroom, South Africab Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatiac Pharmaceutical Chemistry, Potchefstroom University, Potchefstroom, South Africa |
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Abstract: | An improved method of piroxicam benzoate synthesis was described, and an isocratic reversed-phase high-performance liquid chromatography method for its determination was developed and fully validated. The method was found to be specific, precise (relative standard deviation 0.3%), accurate (mean recovery 99.9%), and robust. Limit of detection was estimated at 0.055 µg mL-1 and limit of quantification at 0.185 µg mL-1. The kinetics of piroxicam benzoate hydrolysis in aqueous buffer solutions (pH 1.1 and 10), simulated gastric and intestinal fluids was studied. The hydrolysis followed first-order kinetics. The following rate constants were obtained at pH 10: k = 1.8 × 10-3 hr-1 at 37°C and k = 3.4 × 10-2 hr-1 at 60°C. In acidic media, no significant hydrolysis was observed after 24 hr. During the 24-hr period in simulated intestinal fluid, only 10.9% of the starting ester was hydrolyzed. |
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Keywords: | Piroxicam benzoate Piroxicam HPLC Validation Hydrolysis Kinetics |
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