| 用同步辐射X射线荧光微探针技术研究硝酸镧对单个平滑肌细胞内重要元素分布的影响 |
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| 引用本文： | 韦日生,安丽芝,张京,张凤云,凤志慧,张孙曦,黄宇营,何伟.用同步辐射X射线荧光微探针技术研究硝酸镧对单个平滑肌细胞内重要元素分布的影响[J].核技术,2002,25(10):845-852. |
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| 作者姓名： | 韦日生 安丽芝 张京 张凤云 凤志慧 张孙曦 黄宇营 何伟 |
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| 作者单位： | 1. 北京大学基础医学院生物物理教研室,北京,100083
2. 中国科学院高能物理研究所,北京,100039 |
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| 基金项目： | 国家自然科学基金重大课题 (2 9890 2 80 - 3)，BSRF开放课题 (2 0 0 77)资助 |
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| 摘 要： | 应用同步辐射X射线荧光微探针技术（SRXRF），观察稀土元素化合物硝酸镧La(NO3)3(浓度为10^-8mol/L）对培养的单个大鼠平滑肌细胞内重要元素分布的影响，获得La(NO3)3作用4h,8h,24h时单个细胞内P，K，Ca,Fe,Cu,Zn,La等元素含量的相对计数。实验中观察到，10^-8mol/L La^3 持续作用后，细胞内K，Ca,Fe,Cu,Zn等元素含量分布发生改变。结果表明，La^3 可能富集在细胞内，影响细胞内某些重要元素含量的分布，可能是引起细胞生理生化变化的机制之一。
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| 关 键 词： | 单个平滑肌细胞 元素分布 同步辐射X射线荧光微探针技术 硝酸镧 稀土 生物学效应 生理 生物化学 |
| 修稿时间： | 2002年8月10日 |
Synchrotron radiation X-ray fluorescence (SRXRF) micro-probe investigation of intracellular elements distribution in single smooth muscle cell with La(NO3)3 |
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| WEI Risheng,AN Lizhi,ZHANG Jing,ZHANG Fengyun,FENG Zhihui,ZHANG Sunxi,HUANG Yuying,HE Wei.Synchrotron radiation X-ray fluorescence (SRXRF) micro-probe investigation of intracellular elements distribution in single smooth muscle cell with La(NO3)3[J].Nuclear Techniques,2002,25(10):845-852. |
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| Authors: | WEI Risheng AN Lizhi ZHANG Jing ZHANG Fengyun FENG Zhihui ZHANG Sunxi HUANG Yuying HE Wei |
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| Affiliation: | WEI Risheng 1 AN Lizhi 1 ZHANG Jing 1 ZHANG Fengyun 1 FENG Zhihui 1 ZHANG Sunxi 1 HUANG Yuying 2 HE Wei 2 1 |
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| Abstract: | By using Synchrotron radiation X-ray fluorescence (SRXRF) micro-probe, the relative levels of intracellular important elements (such as K, Ca, Fe, Ni, Cu, Zn, La) in single smooth muscle cell of rat under the actions of 10 -8 mol/L La(NO 3) 3 within 4h, 8h and 24h, were detected respectively. The changes of intracellular important elements distribution in single smooth muscle cell were observed. The accumulation of La 3+ in cell, causing the change of intracellular important elements distribution, is one of the mechanisms of biological effect of rare earth element (REE) in cells. |
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| Keywords: | SRXRF micro-probe La(NO 3) 3 Smooth muscle cell |
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