Glass capillary optics for producing nanometer sized beams and its applications |
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Authors: | Tokihiro Ikeda Yasuyuki KanaiYoshio Iwai Takao M KojimaKazuhiro Maeshima Walter MeisslTomohiro Kobayashi Takuya Nebiki So MiyamotoGrigory P Pokhil Tadashi NarusawaNaoko Imamoto Yasunori Yamazaki |
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Affiliation: | a Atomic Physics Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198 Japanb Cellular Dynamics Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japanc Beam Application Team, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japand Kochi University of Technology, Tosa-Yamada, Kami, Kochi 782-8502, Japane Department of Physics, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japanf D.V. Skobeltsyn Institute of Nuclear Physics, Moscow State University, 119992 Moscow, Russiag Institute of Physics, University of Tokyo, Meguro, Tokyo 153-8902, Japan |
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Abstract: | We have developed a method to produce micro/nano meter sized beams of keV energy highly charged ions (HCIs) and MeV energy protons/He ions with tapered glass capillary optics for application of surface modifications and a biological tool called “cell surgery”, respectively. The transmission through the tapered glass capillaries with inlet diameter of 0.8 mm?, outlet diameter from 900 nm? to several tens of microns and length of about 50 mm was performed using 8/64 keV Ar8+ beams. The transmitted beams had a density enhancement of about 10 and were guided through a capillary tilted by as large as ±100 mrad. The charge state of the beams was kept during the transmission. The combination of MeV proton/He ion beams and the capillary with a thin end window at its outlet can realize pinpoint energy deposition and three-dimensional selection of the bombarding point in an arbitrary position of a living cell or in any liquid object. We demonstrated that a real biological cell, HeLa cell with the nucleus labeled by green fluorescent protein (GFP), was irradiated with the microbeam, which was prepared by 4 MeV He2+ entering a capillary with an end window of 7.3 μm in thickness and outlet diameter of 9.6 μm?. The transmitted MeV ion beams had density enhancement up to 1000 according to the capillary outlet sizes, which are applicable to various material analyses employing microbeams. |
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Keywords: | Micro beam Glass capillary Self-organized charge up Cell irradiation Focusing effect Guiding effect |
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