全谱解卷积核磁共振定量法测定婴幼儿配方乳粉中23种小分子有机物

采用全谱解卷积（Global Spectral Deconvolution，GSD）结合核磁共振定量（Quantitative Nuclear Magneticresonance，qNMR）技术，建立了婴幼儿配方乳粉水提物中核苷酸、核苷、有机酸、氨基酸等23个小分子水溶性物质的含量测定方法。结果显示，所建立GSD-qNMR定量方法的相关系数在0.9960~0.9997之间，精密度的RSD在1.02%~3.72%之间，重复性的RSD在1.34%~3.68%之间，稳定性的RSD在1.62%~3.20%之间，回收率在85.02%~110.00%之间。将该方法胆碱测定值与国标方法进行了对比，结果较为一致（相对标准偏差小于15%）。基于GSD处理的qNMR法各项方法学指标均优于传统qNMR方法，更适用于婴幼儿配方乳粉等复杂食品基质中小分子水溶性物质的定量检测。

Determination of 23 Low-molecular-weight Organic Components Content in Infant Formulas by Quantitative Nuclear Magnetic Resonance with Global Spectral Deconvolution

To establish the quantification method for 23 low-molecular-weight organic components(nucleotides,nucleosides,organic acids and amino acids)in infant formulas,quantitative nuclear magnetic resonance(qNMR)technique was employed based on processing approach of global spectral deconvolution(GSD).The results revealed that it showed a good linear correlation for 23 components in the given concentration ranges(R 2=0.9960~0.9997),with the RSD of precision between 1.02%~3.72%,the RSD of repeatability between 1.34%~3.68%,the RSD of stability between 1.62%~3.20%,and the recovery rate between 85.02%~110.00%.The choline content determined by this method was compared with the national standard method,and the results were consistent(RSD<15%).The results indicated that GSD-qNMR method was better than the traditional qNMR method,and was more suitable for the quantitative detection of low-molecular-weight organic components in complex food matrix such as infant formula.