| ARTP与紫外线复合诱变选育高性能绿僵菌菌株 |
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| 引用本文： | 黄玉,尼玛扎西,薛正莲,张国强. ARTP与紫外线复合诱变选育高性能绿僵菌菌株[J]. 食品工业科技, 2021, 42(4): 60-64,70. DOI: 10.13386/j.issn1002-0306.2020060082 |
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| 作者姓名： | 黄玉 尼玛扎西 薛正莲 张国强 |
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| 作者单位： | 1. 安徽工程大学生物与化学工程学院, 安徽芜湖 241000;2. 西藏自治区农牧科学院农业资源与环境研究所, 西藏拉萨 850000 |
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| 基金项目： | 中央引导地方资金(Y20X20195400004790);平台引进高层次人才资助项目(2018PTJB03);安徽省高校学科拔尖人才项目(jxbjZD22)。 |
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| 摘 要： | 为获得高性能的金龟子绿僵菌菌株,本研究采用常压室温等离子体(Atmospheric and room temperature plasma,ARTP)和紫外线复合诱变处理金龟子绿僵菌,通过比较产孢快慢和生长直径大小进行初筛,用抗紫外能力和毒力作为复筛指标选育菌株,并对育种获得的突变株进行耐热性和遗传稳定性试验,最终在A...
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| 关 键 词： | 金龟子绿僵菌 常压室温等离子体(ARTP) 紫外诱变 复合诱变 筛选 遗传稳定性 |
| 收稿时间： | 2020-06-08 |
Breeding of High Performance Metarhizium anisopliae Strain by ARTP/UV Mutagenesis |
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| HUANG Yu,Nimazhaxi,XUE Zhenglian,ZHANG Guoqiang. Breeding of High Performance Metarhizium anisopliae Strain by ARTP/UV Mutagenesis[J]. Science and Technology of Food Industry, 2021, 42(4): 60-64,70. DOI: 10.13386/j.issn1002-0306.2020060082 |
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| Authors: | HUANG Yu Nimazhaxi XUE Zhenglian ZHANG Guoqiang |
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| Affiliation: | 1. College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China;2. Institute of Resources and Environment, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China |
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| Abstract: | After atmospheric and room temperature plasma(ARTP)and ultraviolet(UV)compound mutation,a high-performance strains of Metarhizium Anisopliae was selected.By comparing the speed of sporulation and the size of growth diameter,selected with the ability of anti-ultraviolet and virulence as the re-screening index,and tested for heat resistance and genetic stability,a good strain AU34 with high sporulation,UV tolerance,strong virulence and stable genetic was selected at the time of ARTP 40 s and UV 120 s.The sporulation time of the strain was faster than the original strain,and the sporulation amount was 1.63±0.22(108 cell/cm^2),which was 63.81%higher than the original strain.After 5 min of UV irradiation,the survival rate of AU34 was 3.18%,which was more resistant to UV irradiation than the original strain.The toxicity of AU34 to Plutella xylostella L.was high.The adjusted mortality rate was 85.71%and LT 50 was 6.12 days.Compared with the original strain,the heat resistance of AU34 was also improved.After subculture for 6 generations,the sporulation of strain AU34 had no obvious change and had good genetic stability. |
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| Keywords: | Metarhizium anisopliae atmospheric and room temperature plasma(ARTP) ultraviolet mutagenesis compound mutation screening genetic stability |
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