响应面法优化黄花鱼鱼鳞脱钙工艺

为了探索黄花鱼鱼鳞脱钙工艺条件，以柠檬酸为脱钙剂，在超声波辅助处理下对黄花鱼鱼鳞脱钙条件进行优化。以脱钙率为评价指标，考察料液比、柠檬酸浓度、超声波时间这3个因素对黄花鱼鱼鳞脱钙的影响，确定单因素最优水平，在此基础上，采用响应面法中的Box-Behnken设计对鱼鳞脱钙工艺条件进行优化并建立数学模型。结果表明，黄花鱼鱼鳞脱钙最佳工艺条件为:料液比1:19（g/mL）、柠檬酸浓度10.5%、超声时间65 min，在此条件下脱钙率高达99.18%。并探讨最优脱钙条件对胶原蛋白损失的影响，结果表明，胶原蛋白损失率为4.08%，损失不大。因此超声波辅助柠檬酸对黄花鱼鱼鳞脱钙有较好的效果，这将为黄花鱼鱼鳞进一步提取胶原蛋白的研究提供基础。

Optimization of Decalcification Process of Yellow Croaker Scales by Response Surface Methodology

In order to explore the decalcification technology of yellow croaker scales,the decalcification condition of the yellow croaker scale was optimized by using citric acid as decalcification agent under the ultrasound-assisted treatment.Three factors including solid-liquid ratio,citric acid concentration and ultrasonic time were selected to investigate the effects of decalcification on yellow croaker scales.The single factor experiment was carried out with the decalcification rate as the evaluation standard to determine the single factor optimal level.On this basis,the Box-Behnken design was adopted to optimize the decalcification of fish scales,and the optimal conditions for decalcification of the yellow croaker scales were obtained as follows,solid-liquid ratio 1∶19(g/mL),citric acid concentration 10.5%,ultrasonic time 65 min,with the demineralization ratio 99.18%.The results also showed that the loss rate of collagen was 4.08%under the optimal conditions,and the loss rate was relatively small.Therefore,the ultrasound-assisted citric acid treatment had a good effect on decalcification of yellow croaker scales,which would provide a basis for further research on extraction of collagen from yellow croaker scales.