TG-DHA高含量脱腥鱼油对脂代谢的调节作用

目的:探究TG-DHA高含量脱腥鱼油的制备及对高脂饮食小鼠脂代谢的调节作用。方法:脂质体包埋技术制备TG-DHA高含量脱腥鱼油。将雄性C57BL/6J小鼠（6周龄）随机分为对照组（C）、模型组（M）、TG-DHA高含量鱼油组（O-DHA）、TG-DHA高含量脱腥鱼油组（L-DHA）。连续灌胃8周后，检测小鼠血清、肝脏脂质水平；测定肝脏脂代谢相关基因脂肪酸合成酶（Fatty acid synthase，FAS）、固醇调节元件结合蛋白1c （Sterol regulatory element binding protein-1c，SREBP-1c）、肉碱棕榈酰转移酶1（Carnitine palmitoyl transferase，CPT1）、脂蛋白脂酶（Lipoprotein lipase，LPL） mRNA表达量。结果:脂质体包埋掩盖了己醛、2，4-庚二烯醛等鱼油中的腥味物质。TG-DHA高含量脱腥鱼油中的DHA含量为73.74%，粒径为159.50 nm，Zeta电位为-43.10 mV，稳定性良好，且在动物体内易于被消化吸收。L-DHA可显著改善高脂饮食小鼠血清和肝脏脂质水平（P<0.05），O-DHA可显著改善高脂饮食小鼠血清水平和肝脏中的TG含量（P<0.05），二者均能极显著降低FAS mRNA表达（P<0.01），显著上调CPT1 mRNA表达（P<0.05），且L-DHA效果更好。结论:TG-DHA高含量脱腥鱼油可改善高脂饮食导致的脂代谢紊乱，其机制与下调肝脏脂肪酸合成，促进脂肪酸分解代谢有关。

Regulation of High Content of TG-DHA Deodorized Fish Oil on Lipid Metabolism

Objective:To investigate the effect of deodorized Triglyceride type-Docosahexaenoic acid(TG-DHA)fish oil on lipid metabolism in mice fed with high-fat diet.Methods:The deodorized TG-DHA fish oil was prepared by liposome embedding technology.Male C57BL/6J mice(6 weeks old)were housed and randomly divided into control group(C),model group(M),TG-DHA fish oil group(O-DHA),deodorized TG-DHA fish oil group(L-DHA).After 8 weeks,blood and liver were collected for lipid accumulation analysis.For gene expression mRNA was extracted from liver for fatty acid synthase(FAS),sterol regulatory element binding protein 1c(SREBP 1c),carnitine palmityl transferase 1(CPt1),lipoprotein lipase(LPL).Results:Liposome embedding could mask the fishy odour in fish oil such as hexanal,2,4-heptadienal.The content of DHA was 73.74%,the particle size was 159.50 nm,and the zeta potential was-43.10 mV.The liposome was stable and easy to be digested and absorbed in animals.L-DHA significantly improved the serum and hepatic lipid levels in mice fed with a high-fat diet(P<0.05),O-DHA significantly improved the serum level and liver TG content of mice fed with high-fat diet(P<0.05).Both of them significantly decreased FAS mRNA expression(P<0.01),and significantly increased CPT1 mRNA expression(P<0.05).The effects of L-DHA seemed to be better.Conclusion:Deodorized TG-DHA fish oil can improve lipid metabolism by high-fat diet.The mechanism is related to the down-regulation of liver fatty acid synthesis and the promotion of fatty acid catabolism.