马铃薯粗蛋白的提取工艺优化及体外抗氧化活性分析

目的:为充分利用马铃薯中的粗蛋白资源,减少资源的浪费和环境污染,优化了马铃薯粗蛋白工艺.方法:采用单因素试验结合响应面法,以马铃薯的粉碎粒度、提取时间、料液比为自变量,以马铃薯粗蛋白含量为响应值,得到马铃薯粗蛋白的优选提取工艺.结果:马铃薯粗蛋白的最佳提取工艺为粒度80目,提取时间4 h,料液比1:25 g/mL,此时...

Extraction Technology Optimization and Antioxidant Activity of Potato Crude Protein in Vitro

Objection:In order to make full use of potato protein resources and reduce the waste of potato protein resources and environmental pollution,the optimum extraction process of potato protein was optimized.Method:The optimum extraction technology of potato protein was obtained by single factor experiment combined with response surface methodology,taking particle size,extraction time,solid-liquid ratio as independent variables and potato protein content as response value.Results:The optimum extraction technology of potato protein was 80 meshes in diameter,4 hours in extraction time and 1∶25 g/mL in solid-liquid ratio.At this time,the content of potato protein was 0.27 mg/g,which was closed to the predicted value 0.28 mg/g.The relative error was 2.56%.In vitro antioxidant experiments showed that potato protein had a strong ability to scavenge ABTS+·,DPPH,·OH.When the concentration was 1 mg/mL,the scavenging rate of potato protein to ABTS reached 92.54%.Conclusion:This process was stable and reliable,and could be used to optimize the extraction of potato protein content in actual industrial production.It would provide a theoretical basis for further development and utilization of potato protein.