姜黄素介导的光动力技术对鲜切马铃薯的杀菌效果

本文运用光动力技术(Photodynamic technology,PDT)对鲜切马铃薯进行非热杀菌,实验选用姜黄素为光敏剂,420 nm LED蓝光为激发光源.探究了光照功率、光照时间、孵育时间以及光敏剂浓度等因素对鲜切马铃薯表面大肠杆菌、金黄色葡萄球菌杀菌效果的影响,并确定最优杀菌条件.结果表明,该技术对大肠杆菌的...

Effects of Curcumin-mediated Photodynamic Technology on Bactericidal Efficacy of Fresh-cut Potatoes

Photodynamic technology(PDT)was used as a kind of non-thermal sterilization in the research to sterilize fresh-cut potatoes.Curcumin was used as photosensitizer in all experiments and the excitation light source is a kind of blue light generated by the light emitting diode(LED),and the wave length was 420 nm in the experiments.In order to determine the optimal bactericidal conditions of PDT against Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)on the surface of fresh-cut potato slices,the bactericidal effects of different light power,light time,incubation time and photosensitizer concentration were detected by counting the number of E.coli and S.aureus after experiments.Results showed that the optimal sterilization conditions against E.coli on the surface of fresh-cut potato slices were:The light power was 40 W,the light time was 20 min,the incubation time was 15 min,and the concentration of curcumin was 30μmol/L;the optimal sterilization conditions against S.aureus were:The light power was 20 W,the light time was 10 min,the incubation time was 15 min,and the concentration of curcumin was 30μmol/L.Compared with the control group,the total number of bacterial colonies of E.coli and S.aureus after being treated by PDT were reduced by 3.60 and 5.23 lg CFU/mL,respectively.