三苯胺类荧光探针的合成与细胞成像

以三苯胺为原料,通过Vilsmeier-Haack和McMurry反应合成了一个碳碳双键连接两个三苯胺的荧光分子1,2-二(三苯胺基)乙烯(TPAS)。采用紫外-可见光谱和荧光光谱对TPAS的光学性质进行了考察。结果表明:TPAS有很强的荧光发射性能,荧光发射为蓝光,荧光强度与浓度的线性关系较好,且溶剂化效应不强,光稳定性良好。采用扫描电镜测试了TPAS的聚集态行为。结果显示:当水的体积分数增大后,TPAS分子聚集,形貌改变。采用MTT法对TPAS进行了细胞毒性测试。结果表明,不同TPAS浓度下,细胞的存活度都在80%以上,说明TPAS对细胞的毒性很低,生物相容性好。并实现了TPAS对A549的活细胞成像。

Synthesis and Cell Imaging of Triphenylamine-based Fluorescent Probe

In this paper, taking triphenylamine as a raw material, 4,4''-(ethene-1,2-diyl)bis (N,N-diphenylaniline) (TPAS) has been synthesized, which was composed by C=C and two triphenylamine groups via Vilsmeier-Haack reaction and McMurry reaction. The optical performance of TPAS has been studied by UV-Vis absorbance spectrum and fluorescence spectrum. The results showed that TPAS had strong fluorescence emission, and the fluorescence emission was blue light. The fluorescence intensity had a good linear relationship with the concentration, and the solvation effect was not strong, and the optical stability was good. The aggregation of TPAS has been characterized by scanning electron microscope(SEM). And the results showed that when the volume fraction of water increased, the TPAS molecules accumulated and the morphology changed. The Cytotoxicity of TPAS was tested by MTT assays, the cell viability was above 80% with the increase of TPAS concentration in the text, which was revealed that TPAS is biocompatible and have low toxicity to cells. Then achieved imaging of A549.