Sweet potato (Ipomoea batatas [L.] Lam ‘Tainong 57’) storage root mucilage with antioxidant activities in vitro |
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Authors: | Dong-Jiann Huang Hsien-Jung Chen Wen-chi Hou Chun-Der Lin Yaw-Huei Lin |
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Affiliation: | 1. Institute of Botany, Academia Sinica, Nankang, Taipei 11529, Taiwan;2. Department of Horticulture, Chinese Culture University, Taipei 111, Taiwan;3. Graduate Institute of Pharmacognosy Science, Taipei Medical University, Taipei 110, Taiwan |
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Abstract: | Sweet potato storage root mucilage was extracted and purified by SDS and heating treatments. Total antioxidant activity, DPPH (1,1-diphenyl-2-picrylhydrazyl) staining, reducing power method, metal ion-dependent hydroxyl radical, FTC (ferric thiocyanate) method, and protection of calf thymus DNA against hydroxyl radical-induced damage were studied. Half-inhibition concentrations, IC50, were 0.08 mg/ml and IC50 > 0.1 mg/ml, respectively, for the crude and purified mucilage in the total antioxidant activity test. In the DPPH staining, the crude and purified mucilage appeared as white spots when they were diluted to 50 and 100 μg per application, respectively. Like total antioxidant activity, reducing power, scavenging capacity against hydroxyl radical, FTC activity and protection of calf thymus DNA against hydroxyl radical-induced damage were found in the mucilage. It is suggested that the mucilage might contribute its antioxidant activities against both hydroxyl and peroxyl radicals. |
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Keywords: | Sweet potato Antioxidant Mucilage Hydroxyl radical Peroxyl radical 1 1-Diphenyl-2- picryl hydrazyl (DPPH) |
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