Tyr-Pro-Lys,an angiotensin I-converting enzyme inhibitory peptide derived from broccoli (Brassica oleracea Italica) |
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Authors: | Ju-Eun Lee In Young BaeHyeon Gyu Lee Cha-Bum Yang |
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Affiliation: | Department of Food and Nutrition, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea |
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Abstract: | To investigate the naturally occurring angiotensin I-converting enzyme (ACE) inhibitor, broccoli (Brassica oleracea Italica) extracts were used for its isolation and identification. After treatment with 50% acetone for membrane breakdown, ethyl acetate, n-butanol, and water were used for the preparation of broccoli extracts. The water-soluble extract from broccoli had 76.9% ACE inhibitory activity, while those of other organic solvent extracts showed lower ACE inhibitory activities. An ACE inhibitory peptide was isolated using column chromatographic methods including: Amberlite XAD-4, Sephadex LH-20, and high performance liquid chromatography. The purified ACE inhibitory peptide was identified to be a tripeptide, Tyr-Pro-Lys, having an IC50 value of 10.5 μg protein/ml. |
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Keywords: | Broccoli Brassica oleracea Italica Angiotensin I-converting enzyme inhibitor Bioactive peptide |
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