Enzymatic proteolysis,under high pressure of soybean whey: Analysis of peptides and the allergen Gly m 1 in the hydrolysates |
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Authors: | Elena Peñ as,Guadalupe Pré stamo,Florentino Polo,Rosario Gomez |
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Affiliation: | 1. Departamento de Ciencia y Tecnologia de Productos Vegetales, Instituto del Frío, Ciudad Universitaria, c/José Antonio Nováis 10, 28040-Madrid, Spain;2. Departamento de Ciencia y Tecnologia de Productos Lácteos, Instituto del Frío, Ciudad Universitaria, c/José Antonio Nováis 10, 28040-Madrid, Spain;3. Alk-Abello S.A., Alergia e Inmunología Miguel Fleta 19, 28037 Madrid, Spain |
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Abstract: | Soybean (Glycine max) whey was hydrolyzed with Alcalase, Neutrase, Corolase 7089 and Corolase PNL during high pressure (HP) treatment at 100, 200 and 300 MPa and at atmospheric pressure for 15 min. The protein content and the degree of hydrolysis were determined. Furthermore, the allergen Gly m 1 in the treated soybean whey and the hydrolysates was detected. The results showed that HP treatments increased the hydrolysis by the four proteases used. Pressure at 200 and 300 MPa proved to be better pressures to enhance the proteolysis. The immunochemical response of soybean whey to anti-Gly m 1 monoclonal antibodies decreased after the HP treatments and this decrease was greater after the combined treatment of high pressure and enzymatic hydrolysis. Soybean whey proteins hydrolysed at high pressure could be used as sources of peptides with low antigenicity when incorporated as food ingredients. |
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Keywords: | Allergy Glycine max Soy-whey Gly m 1 High pressure Hydrolysis |
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