Hydrogen production from Chlamydomonas reinhardtii biomass using a two-step conversion process: Anaerobic conversion and photosynthetic fermentation |
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Authors: | Mi-Sun Kim Jin-Sook Baek Young-Su Yun Sang Jun Sim Sunghun Park Sun-Chang Kim |
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Affiliation: | 1. Biomass Research Center, Korea Institute of Energy Research, 71-2 Jang-dong, Yuseong-gu, Daejeon 305-343, Republic of Korea;2. Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea;3. Department of Chemical Engineering, Pusan National University, Pusan 609-735, Republic of Korea;4. Department of Biological Science, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea |
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Abstract: | Chlamydomonas reinhardtii UTEX 90 accumulated 1.45 g dry cell weight and 0.77 g starch/L during photosynthetic growth using TAP media at 25 °C in presence of 2% CO2 for 3 days. C. reinhardtii biomass was concentrated and then converted into hydrogen and organic acids by anaerobic fermentation with Clostridium butyricum. Organic acids in the fermentate of algal biomass were consecutively photo-dissimilated to hydrogen by Rhodobacter sphaeroides KD131. In the concentrated algal biomass 52% of the starch was hydrolyzed to 37.1 mmol H2/L-concentrated algal biomass and 13.6, 25.5, 7.4 and 493 mM of formate, acetate, propionate, and butyrate, respectively by C. butyricum. R. sphaeroides KD131 evolved 5.72 mmol H2 per ml-fermentate of algal biomass under illumination of 8 klux at 30 °C. Only 80% of the organic acids, mainly butyrate, were hydrolyzed during photo-incubation. During anaerobic conversion, 2.58 mol H2/mol starch–glucose was evolved using C. butyricum and then 5.72 mol H2/L-anaerobic fermentate was produced by R. sphaeroides KD131. Thus, the two-step conversion process produced 8.30 mol H2 from 1 mol starch–glucose equivalent algal biomass via organic acids. |
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Keywords: | Algal biomass C. reinhardtii Hydrogen energy C. butyricum R. sphaeroides |
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