Biochemistry of hydrogen metabolism in Chlamydomonas reinhardtii wild type and a Rubisco-less mutant

Sulfate nutrient-deprivation in Chlamydomonas reinhardtii   brings about prompt degradation of Rubisco and a concomitant substantial accumulation of starch. These changes precede hydrogen (_H2${\mathrm{H}}_2$) photoevolution by the cells. The cause-and-effect relationship between Rubisco loss, starch accumulation and subsequent _H2${\mathrm{H}}_2$-photoevolution in C. reinhardtii, and the role of illumination for these changes to occur, was investigated in this work. A Rubisco-less and acetate-requiring mutant of C. reinhardtii   (CC2653) was employed as a tool in this investigation and compared to the wild type (WT) in terms of protein and starch metabolic flux and _H2${\mathrm{H}}_2$-evolution upon sulfur deprivation. Results showed a prompt Rubisco degradation and concomitant 10-fold starch accumulation in the WT in the light, which was completed within 48 h of S-deprivation. This was followed by a regulated starch degradation and concomitant _H2${\mathrm{H}}_2$-photoevolution, which lasted for up to 120 h in S-deprivation. This massive flux of primary metabolites (protein and starch) did not occur in the dark in the WT, suggesting a strictly light-dependent and integrated process in metabolite rearrangement and _H2${\mathrm{H}}_2$-photoevolution in C. reinhardtii  . The Rubisco-less CC2653 mutant failed to accumulate starch upon S-deprivation in the light or dark and also failed to evolve _H2${\mathrm{H}}_2$ gas. These results suggested a temporal cause-and-effect relationship between the light-dependent catabolism of Rubisco and starch accumulation, and the subsequent ability of the cell to perform a light-dependent starch degradation and _H2${\mathrm{H}}_2$-photoevolution. The regulated starch breakdown in the light apparently provides the endogenous substrate that supports _H2${\mathrm{H}}_2$-evolution, both by feeding electrons into the plastoquinone pool in chloroplasts, and indirectly by sustaining mitochondrial respiration for the maintenance of anaerobiosis in the cell.