| 分子生物学在油田微生物多样性研究中的应用进展 |
| |
| 引用本文： | 庄文.分子生物学在油田微生物多样性研究中的应用进展[J].山东化工,2010,39(5):24-26,30. |
| |
| 作者姓名： | 庄文 |
| |
| 作者单位： | 青岛科技大学生命科学研究所,山东,青岛,266042 |
| |
| 摘 要： | 介绍了近几年国内外在油田微生物研究中应用最广泛的一些分子生物学技术,它们基于16S rRNA,如16S rRNA测序,变性梯度凝胶电泳(DGGE),末端限制性片段长度多态性分析(T-RFLP)等,并分别指出了其优缺点。同时还介绍了一些油田研究中最受关注的微生物。
|
| 关 键 词： | 分子生物学 油田 16S rRNA 微生物多样性 |
Applications of Molecular Biology in Oil Field Microbial Biodiversity Research |
| |
| ZHUANG Wen.Applications of Molecular Biology in Oil Field Microbial Biodiversity Research[J].Shandong Chemical Industry,2010,39(5):24-26,30. |
| |
| Authors: | ZHUANG Wen |
| |
| Affiliation: | ZHUANG Wen ( Institute for Life Sciences, Qingdao University of Science ＆ Technology ( QUST), Qingdao 266042, China) |
| |
| Abstract: | Described the most commonly used molecular biology techniques in oil field microorganisms research at home and abroad. They were based mainly on 16S rRNA, such as 16S rRNA sequencing, denaturant gradient gel electrophoresis （ DGGE）, terminal restriction fragment length polymorphism （T - RFLP）. Pointed out their advantages and disadvantages respectively, and introduced some of the micro - organisms which were of largest concentration in research. |
| |
| Keywords: | 16S rRNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
